COVID-19: Measured from 1 drop of blood in 30 minutes: Type and amount of antibody
RIKEN:
Developed “virus / microarray detection system”.
Against the new coronavirus
The type and amount of antibody,
It can be measured in 30 minutes from a drop of blood.
It has 500 times the sensitivity of immunochromatography, which is a simple test.
Medical Technology News-MONOist
https://monoist.atmarkit.co.jp/mn/articles/2109/22/news017.html
Measuring new coronavirus antibody “on the spot” | RIKEN
https://www.riken.jp/press/2021/20210903_2/
SARS-CoV-2 proteins microarray by photoimmobilization for serodiagnosis of the antibodies
An automated microarray diagnostic system for SARS-CoV-2 protein-specific antibodies
was developed using photoimmobilized viral proteins.
Photoimmobilization strategy
was used to immobilize proteins by crosslinking reactions of photoreactive polymers.Aqueous solutions of proteins
were spotted on a polymer-coated plate and dried in the air.Then, the proteins
were immobilized via irradiation with an ultraviolet lamp.Virus-specific IgG and IgM in the serum
were automatically assayed using the microarray plate.After the serum was added on the microarray plate,
peroxidase-conjugated anti-human-IgG or -IgM antibody was added,
and the chemical luminescence intensity was detected using a sensitive charge-coupled device camera.
The developed microarray assay
showed higher sensitivity than the conventional immune chromatography. Additionally, compared with conventional microarray using nitrocellulose slide,
the developed microarray format
was useful for rapid assay formats in serodiagnosis of viral infectious diseases and assessment of immune status for clinical applications.
Bulletin of the Chemical Society of Japan