COVID-19：在 30 分钟内从 1 滴血中测量：抗体的类型和数量
理研：
开发“病毒/微阵列检测系统”。
对抗新型冠状病毒
抗体的种类和数量，
它可以在 30 分钟内从一滴血中测量出来。
它的灵敏度是免疫层析的 500 倍，这是一项简单的测试。
医疗技术新闻-MONOist
https://monoist.atmarkit.co.jp/mn/articles/2109/22/news017.html
“现场”测量新冠病毒抗体 | RIKEN
https://www.riken.jp/press/2021/20210903_2/
SARS-CoV-2 proteins microarray by photoimmobilization for serodiagnosis of the antibodies
An automated microarray diagnostic system for SARS-CoV-2 protein-specific antibodies
was developed using photoimmobilized viral proteins.
Photoimmobilization strategy
was used to immobilize proteins by crosslinking reactions of photoreactive polymers.Aqueous solutions of proteins
were spotted on a polymer-coated plate and dried in the air.Then, the proteins
were immobilized via irradiation with an ultraviolet lamp.Virus-specific IgG and IgM in the serum
were automatically assayed using the microarray plate.After the serum was added on the microarray plate,
peroxidase-conjugated anti-human-IgG or -IgM antibody was added,
and the chemical luminescence intensity was detected using a sensitive charge-coupled device camera.
The developed microarray assay
showed higher sensitivity than the conventional immune chromatography. Additionally, compared with conventional microarray using nitrocellulose slide,
the developed microarray format
was useful for rapid assay formats in serodiagnosis of viral infectious diseases and assessment of immune status for clinical applications.
Bulletin of the Chemical Society of Japan