COVID-19: University of Tokyo, corona diagnosis in one hour: genome editing technology CRISPR-Cas3
COVID-19:
Institute of Medical Science, Tokyo University:
On June 3, the University of Tokyo announced “a new method for corona diagnosis in one hour”.
A method that applies genome editing technology that modifies genes.
You can check the new coronavirus / infection status in 1 hour.
Shorter time than PCR method:
Results found in less time than PCR
Higher accuracy than the antigen test
It is suitable for “on-site use where accuracy is required”.
Prof. Tomoshi Mashita (Animal Genetics):
Prof. Tomoshi Mashita of the research team says that it can be used in quarantine and fever outpatients.
KYODO
https://this.kiji.is/640866088961705057
Development of rapid diagnostic method for COVID-19 using domestic genome editing technology CRISPR-Cas3
–Able to make accurate diagnosis with test strips in a minimum of 40 minutes-(announced at medRxiv)
Now, there is a need for a new COVID-19 rapid diagnostic method that can be used quickly and easily in the medical field.
Institute of Medical Science, University of Tokyo: Genome Research Team
Utilizes domestic genome editing technology CRISPR-Cas3 (Note 1).
Established as a rapid diagnostic method for COVID-19, “developing a method (CONAN method) for accurately detecting a minute amount of viral RNA”.
Using the CRISPR test, you can detect the presence of tens (tens of copies) of viral RNA with a test strip in as little as 40 minutes.
COVID-19 sample: inspection results
Results using samples from COVID-19 patients,
Positive agreement rate (PPA): 90% (9/10 cases),
The negative agreement rate (NPA) was 95.3% (20/21 cases).
It was confirmed that the detection sensitivity and detection specificity were almost the same as the PCR test method.
This CRISPR test:
This can be done with common reagents, test papers, and a heat retaining device (Note 3).
It is thought that it will be a cheap, quick, and easy-to-diagnose POCT (Note 4).
Institute of Medical Science, University of Tokyo
https://www.ims.u-tokyo.ac.jp/imsut/jp/research/papers/post_135_00016.html
Rapid and accurate detection of novel coronavirus SARS-CoV-2 using CRISPR-Cas3
Abstract
Novel coronavirus SARS-CoV-2 outbreaks
have rapidly spread to multiple countries, highlighting the urgent necessity for fast, sensitive, and specific diagnostic tools for virus surveillance.
Here, the previously unknown collateral single-stranded DNA cleavage we observed with type I CRISPR-Cas3 highlights its potential for development as a Cas3-mediated rapid (within 40 min), low-cost, instrument-free detection method for SARS-CoV-2.
This Cas3-based assay
is comparable with Cas12- and real-time reverse-transcriptase PCR-based assays in its speed and sensitivity, but offers greater specificity for single-base-pair discrimination while negating the need for highly trained operators.
These findings
support the use of CRISPR diagnostics for point-of-care testing in patients with suspected SARS-CoV-2 infections.
medRxiv
https://www.medrxiv.org/content/10.1101/2020.06.02.20119875v1