Shine all the proteins in the cell
This time, the international collaborative research team aims to analyze proteome with ultra-high sensitivity (single molecule sensitivity) · low cost, by using a labeling method of labeling almost all kinds of proteins with fluorescent dyes and a labeling method of “single molecule fluorescence imaging method [5] “was applied to evaluate the labeling ratio.
Application of this method to human cultured cells confirmed that proteins of various sizes can be fluorescently labeled with high efficiency of 50 to 90%.
This research was published on the online version (dated 5th July) prior to publication of the scientific journal “Bioconjugate Chemistry” in the United States.
RIKEN